Journal: Science Advances

Article Title: Designer cellular spheroids with DNA origami for drug screening

doi: 10.1126/sciadv.ado9880

Figure Lengend Snippet: ( A ) Schematic of the high-throughput AI screening platform for identifying antifibrotic candidate molecules using fibrosis package NAC-Livers. ( B ) Representative high-content images of fibrotic pNAC-Livers treated with 37 candidate molecules, where PHHs were labeled with GFP (green) and HSCs were labeled with RFP (red). See fig. S21A for additional information on the locations and concentrations of 37 candidate molecules. n = 3 independent experiments. ( C and D ) Heatmap showing the fluorescence intensity of GFP (C) and RFP (D) in high-content imaging of each pNAC-Liver in (B). ( E ) The binding pose of axitinib (candidate 4) with PDK1. ( F ) H&E, Sirius Red, and α-SMA staining of mouse liver sections from three treatment groups: negative control (NC), DDC Ctrl, and axitinib. NC represents the group with normal diet, DDC Ctrl represents the group with 0.1% DDC diet modeling and intraperitoneal injection of solvent [saline with 2% dimethyl sulfoxide (DMSO) + 30% polyethylene glycol 300 + 2% Tween 80], and axitinib represents the group fed with 0.1% DDC diet and intraperitoneal injection of axitinib. See table S2 for the concentration for in vivo administration. ( G and H ) Quantification of Sirius Red–positive area (G) and the H-score in α-SMA immunohistochemical staining slices (H) of each group in (F). ( I and J ) The concentrations of serum aspartate aminotransferase (AST) (I) and alanine aminotransferase (ALT) (J) in mouse serum of each group in (F). Data in (G) to (J) are mean ± SD of n = 3 independent experiments.

Article Snippet: All small molecules used in hepatotoxicity tests and high-content drug screening were purchased from TopScience.

Techniques: High Throughput Screening Assay, Labeling, Fluorescence, Imaging, Binding Assay, Staining, Negative Control, Injection, Solvent, Saline, Concentration Assay, In Vivo, Immunohistochemical staining

Journal: BioMed Research International

Article Title: Role of the Death Receptor and Endoplasmic Reticulum Stress Signaling Pathways in Polyphyllin I-Regulated Apoptosis of Human Hepatocellular Carcinoma HepG2 Cells

doi: 10.1155/2018/5241941

Figure Lengend Snippet: Primer sequences and Tm for RT-qPCR.

Article Snippet: The primary antibodies included B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X (Bax), IRE-1, FAS associated via death domain (FADD), FAS, β -actin (Wuhan Boster Biological Technology, Ltd.,), TNF- α , CHOP and caspase-12 (BIOSS, Beijing, China) at 1:500 dilution and Cleaved-caspase3 (Cell Signaling Technology, Inc., Danvers, MA, USA), cleaved-caspase8 (Beyotime Institute of Biotechnology, Shanghai, China) at 1:1 000 dilution.

Techniques: Sequencing

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Scutellarein selectively targets multiple myeloma cells by increasing mitochondrial superoxide production and activating intrinsic apoptosis pathway.

doi: 10.1016/j.biopha.2018.09.024

Figure Lengend Snippet: Fig. 2. Scutellarein treatment activated the intrinsic apoptosis pathway in multiple myeloma cells in vitro. A–C, analysis of caspase-9, -8 and -3 activities in IM-9, MM.1R or viable circulating B lymphocytes (CBL) after treatment with indicated concentrations of scutellarein for 24 h. Caspase activity was measured by the relative fluorescent units (RFU) of cleaved substrates. Data was normalized to the vehicle-treated CBL group. D–I, influence of FADD or APAF1 knockdown on the apoptosis- inducing effect of 400 μg/ml scutellarein treatment for 24 h. shRNA targeting green fluorescent protein gene (sh-NC) was used as negative control for FADD or APAF1 knockdown. Data in DeI were normalized to the un-transfected group (WT) in each cell line. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Antibodies for detecting FADD (PA1464, BosterBio, Pleasanton, USA), Apaf-1 (PA1249-1, BosterBio), active Caspase-3 (269518, Novus Biologicals, Littleton, USA) Bax (AF820, R& D Systems, Minneapolis, USA), Bcl-2 (RP1003, BosterBio) and GAPDH (HPA040067, Atlas Antibodies, Bromma, Sweden) were applied following manufacturer’s instructions.

Techniques: In Vitro, Activity Assay, Knockdown, shRNA, Negative Control, Transfection